Search results for " Swab"
showing 10 items of 19 documents
Description of microsatellite markers and genotyping performances using feathers and buccal swabs for the Ivory gull (Pagophila eburnea)
2011
We report 22 new polymorphic microsatellites for the Ivory gull (Pagophila eburnea), and we describe how they can be efficiently co-amplified using multiplexed polymerase chain reactions. In addition, we report DNA concentration, amplification success, rates of genotyping errors and the number of genotyping repetitions required to obtain reliable data with three types of noninvasive or nondestructive samples: shed feathers collected in colonies, feathers plucked from living individuals and buccal swabs. In two populations from Greenland (n = 21) and Russia (Severnaya Zemlya Archipelago, n = 21), the number of alleles per locus varied between 2 and 17, and expected heterozygosity per populat…
Esophageal atresia and Beckwith–Wiedemann syndrome in one of the naturally conceived discordant newborn twins: first report
2018
Key Clinical Message Recent studies report a high incidence of monozygotic twinning in Beckwith–Wiedemann syndrome. A phenotypical discordance in monozygotic twins is rare. Twinning and Beckwith–Wiedemann syndrome show higher incidence in children born after assisted reproductive techniques. We report on the first observation of esophageal atresia and Beckwith–Wiedemann syndrome in one of the naturally conceived discordant monozygotic twins.
Frequency of micronucleus in oral epithelial cells after exposure to mate-tea in healthy humans
2014
Objectives: The aim of this study was to evaluate the possibility of technique simplification for cytology slides in order to evaluate the frequency of micronuclei (FMic) and conduct a experiment looking to know the FMic of oral epithelial cells of healthy volunteers exposed to mate tea (Ilex paraguarariensis). Material and Methods: This is a laboratorial and nonrandomized trial (quasi-experiment), where the nonusers subjects were exposed to mate-tea, consumed in the traditional way, two drinks, two times a day for a single week. Two cytology of exfoliated epithelial cells were obtained before and after the mate tea exposition. Results: The sample was composed by 10 volunteers. The age rang…
Nasal Cellularity in 183 Unselected Schoolchildren Aged 9 to 11 Years
2002
Objective. Although rhinitis is extremely frequent in children, methods for assessing the severity of nasal inflammation produce results with wide variability and hence weak clinical significance. We designed this epidemiologic investigation to define the clinical usefulness of assessing nasal cellularity in children. Methods. We studied 183 of 203 eligible unselected schoolchildren who were aged 9 to 11 years and whose parents gave informed consent and completed a questionnaire on the history of atopic and respiratory symptoms. In all children, nasal swabs were obtained from both nostrils and eluted in saline and slides were prepared from cytospin preparations for staining and white cell c…
Quantification of fluorescent STR genotyping results for chimerism control after bone marrow transplantation
2003
1. IntroductionEngraftment of donor stem cells after allogeneic bone marrow transplantation can begenetically monitored by PCR typing of DNA polymorphisms [1]. Successful engraftmentwith complete chimerism and presence of the donor’s genotype in the bone marrow has tobe demonstrated, and the presence of the patient’s alleles has to be excluded. Detection ofthe patient’s alleles provides evidence for an incomplete chimerism or for a relapse ofmalignant disease. STRs have been used successfully for this type of genetic monitoring[2]. For the present study, we have developed an approach to quantify the ratio of donorchimerism using mock mixture experiments. The usefulness of our approach is de…
The burden of Candida species colonization in NICU patients: a colonization surveillance study
2017
Fungal infections are an important cause of morbidity and mortality in neonatal intensive care units (NICUs). The identifi-cation of specific risk factors supports pre-vention of candidemia in neonates. Effec-tive prophylactic strategies have recently become available, but the identification and adequate management of high-risk in-fants is still a priority. Prior colonization is a key risk factor for candidemia. For this reason, surveillance studies to monitor in-cidence, species distribution, and antifun-gal susceptibility profiles, are mandatory. Among 520 infants admitted to our NICU between January 2013 and December 2014, 472 (90.77%) were included in the study. Forty-eight out of 472 (…
Recensione a: La porta del sapere. Cultura alla corte di Federico II di Svevia, Roma, Carocci, 2019
2020
Recensione a Fulvio Delle Donne, La porta del sapere. Cultura alla corte di Federico II di Svevia, Roma, Carocci, 2019, pp. 270. Il volume esamina le varie forme di cultura che si svilupparono alla corte di Federico II di Svevia (produzione letteraria in latino e in volgare, produzione artistica e giuridica, etc.). Review to Fulvio Delle Donne, The door to knowledge. Culture at the court of Frederick II of Swabia, Rome, Carocci, 2019, pp. 270. The volume examines the various forms of culture that developed at the court of Frederick II of Swabia (literary production in Latin and in the vernacular, artistic and legal production, etc.).
Orgoglio poetico e lode del sovrano nei “carmina” di Enrico di Avranches per Federico II
2021
Nel 1878 Eduard Winkelmann pubblicò in «Forschungen zur deutschen Geschichte», tre carmina attribuibili a Enrico di Avranches, poeta normanno del XIII secolo. I tre componimeni sono trasmessi – come la stragrande maggioranza dei carmina di Enrico – dal ms. Dd. XI 78 della University Library di Cambridge, esemplato nel secolo XIII e appartenuto a Matteo Paris (siglum A). Questo intervento presenta una prima sezione dedicata alla presentazione della figura di Enrico di Avranches, con una rassegna bibliografica delle sue opere principali, alla quale segue una seconda e più ampia parte dedicata all’analisi dei tre carmina per Federico II, studiati nelle loro caratteristiche contenutistiche e fo…
Results of a collaborative study of the EDNAP group regarding mitochondrial DNA heteroplasmy and segregation in hair shafts.
2004
Abstract A collaborative exercise was carried out by the European DNA Profiling Group (EDNAP) in order to evaluate the distribution of mitochondrial DNA (mtDNA) heteroplasmy amongst the hairs of an individual who displays point heteroplasmy in blood and buccal cells. A second aim of the exercise was to study reproducibility of mtDNA sequencing of hairs between laboratories using differing chemistries, further to the first mtDNA reproducibility study carried out by the EDNAP group [Forensic Sci. Int. 97 (1998) 165]. Laboratories were asked to type 2 sections from each of 10 hairs, such that each hair was typed by at least two laboratories. Ten laboratories participated in the study, and a to…
Corynebacterium macginleyi isolation from conjunctival swab in Italy
2002
Corynebacterium macginleyi was isolated from conjunctival swabs of a farmer suffering from purulent conjunctivitis. This species has only recently been reported in Switzerland and Germany to be exclusively isolated from ocular surfaces. This represents the first isolation of C. macginleyi in Italy indicating that its circulation is not geographically limited. © 2002 Elsevier Science Inc. All rights reserved.